• Users Online: 480
  • Print this page
  • Email this page
Year : 2022  |  Volume : 19  |  Issue : 4  |  Page : 721-728

Separation, purification, and phylogenetic characterization of detergent-compatible protease produced by Penicillium species

College of Health and Medical Technologies, Middle Technical University, Baghdad, Iraq

Correspondence Address:
Omar Sadik Shalal
College of Health and Medical Technologies, Middle Technical University, Baghdad
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/MJBL.MJBL_232_22

Rights and Permissions

Background: Filamentous fungal forms are said to secrete multiple peptidases with portent detergent capabilities in addition to their usage in food, beverages, and pharmaceutical industries. Objective: The current study isolated and purified fungal isolates from clinical samples and studied their capability as caseinolytic and in removing blood stains from fabrics. Materials and Methods: The present work isolated nine isolates, which are protease-positive and out of them eight belong to Penicillium sp. From the IS2 region, amplification of the isolates concluded their match using Blastn as Penicillium citrinum in majority. Results: The enzyme extract (P18), which was found effective in removing blood stains with good caseinolytic activity, was found to belong to P. citrinum as from Blastn studies. In addition, these proteases extracted were highly compatible with commercially available detergents. P4 was found to remove blood stains from fabrics in less than 30 min (P<0.02) when compared with other extracts (P1 and P4). Conclusion: The ability to secrete protease in promising amounts along with its potential usage as detergent makes these enzymes useful in industries, especially for the laundry industry.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded16    
    Comments [Add]    

Recommend this journal