• Users Online: 8
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2021  |  Volume : 18  |  Issue : 4  |  Page : 351-357

Evaluation of glycated hemoglobin results in different anticoagulant materials and methods


1 Basic Sciences Department, College of Dentistry, Babylon University, Babylon, Iraq
2 Biochemistry Department, College of Medicine, Baghdad University, Baghdad, Iraq

Correspondence Address:
Ammar Hatem Abdullateef
Basic Sciences Department, College of Dentistry, Babylon University, Babylon.
Iraq
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/MJBL.MJBL_49_21

Get Permissions

Background: Samples for glycated hemoglobin (HbA1c) measurements should be collected in ethylenediaminetetraacetic acid (EDTA) tubes, and only a few studies were available for the assessment of its measurements by another anticoagulant tube. There are different methods with different principles for the measurement of HbA1c in addition to high-performance liquid chromatography (HPLC) standard method, and one of these methods is ion exchange chromatography (IEC) which depends on the charge differences principle. Aim of the study: The aim is to compare the effect of different anticoagulant additives (EDTA, lithium heparin, and sodium citrate) on HbA1c value and to compare the results of HbA1c obtained by the IEC method with those by the HPLC method. Materials and Methods: This case methodology study investigated the effect of different anticoagulant additives on HbA1c values on 40 diabetic patients and compared the results of HbA1c values between HPLC method and IEC method on another 40 diabetic patients. Results: The study showed that there were non-significant differences in HbA1c mean values among different types of these anticoagulant materials and non-significant differences in glycated hemoglobin mean values between the methods of HPLC and IEC, and sensitivity and specificity for these techniques were 97.3% and 100% and 97.1% and 100%, respectively. Conclusion: This study showed that HbA1c can be tested with lithium heparin or sodium citrate as an alternative to EDTA tube, and the IEC method can be used as one of the main methods for the assessment of HbA1c.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed80    
    Printed0    
    Emailed0    
    PDF Downloaded11    
    Comments [Add]    

Recommend this journal